Optimum storage conditions for produkt of transiently expressed epitopes of Human papillomavirus using Potato virus X-based vector

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F08%3A26073" target="_blank" >RIV/60460709:41210/08:26073 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Optimum storage conditions for produkt of transiently expressed epitopes of Human papillomavirus using Potato virus X-based vector

Original language description

We describe the optimized conditions of recombinant Potato virus A coat protein (ACP) carrying two different epitopes from Human papillomavirus type 16 (HPV-16). Epitope derived from minor capsid protein L2 was expressed as N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was fused to its C-terminal. The construct was cloned into Potato X potexvirus (PVX) based vector and transiently expressed in plants using Agrobacterium tumefaciens mediated inoculation. The effect of storage conditions on the serological activity of L2ACPE7 was studied by ELISA using IgG anti PVX, PVA and L2. Purified L2ACPE7 stored freeze-dried (at -20oC), frozen at various temperatures (-20oC,-70 oC) and at +4oC wer tested. Purified L2ACPE7 was most stable as lyophilized material stored at -20oC. Our study demonstrates suitable way for the storage of plant materiál containing foreign viral epitopes for the purposes of edible vaccination.

Czech name

Optimální podmínky uchovávání produktu transientně exprimovaných epitopů lidského papilloma viru pomocí virového vektoru odvozeného od X viru bramboru

Czech description

We describe the optimized conditions of recombinant Potato virus A coat protein (ACP) carrying two different epitopes from Human papillomavirus type 16 (HPV-16). Epitope derived from minor capsid protein L2 was expressed as N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was fused to its C-terminal. The construct was cloned into Potato X potexvirus (PVX) based vector and transiently expressed in plants using Agrobacterium tumefaciens mediated inoculation. The effect of storage conditions on the serological activity of L2ACPE7 was studied by ELISA using IgG anti PVX, PVA and L2. Purified L2ACPE7 stored freeze-dried (at -20oC), frozen at various temperatures (-20oC,-70 oC) and at +4oC wer tested. Purified L2ACPE7 was most stable as lyophilized material stored at -20oC. Our study demonstrates suitable way for the storage of plant materiál containing foreign viral epitopes for the purposes of edible vaccination.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologia Plantarum

ISSN

0006-3134

e-ISSN

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Volume of the periodical

50

Issue of the periodical within the volume

52

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

3

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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