Transient expression of fusion gene coding for the HPV-16 epitopes fused to the sequence of potyvirus coat protein using different means of inoculation of Nicotiana benthamiana and Brassica rapa, cv. Rapa plants.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F08%3A26756" target="_blank" >RIV/60460709:41210/08:26756 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/08:00320736

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Transient expression of fusion gene coding for the HPV-16 epitopes fused to the sequence of potyvirus coat protein using different means of inoculation of Nicotiana benthamiana and Brassica rapa, cv. Rapa plants.

Original language description

We describe the effect of the different means of inoculation of Nicotina benthamiana plants on the amount of transiently expressed fussion gene coding for the Human papillomavirus type 16 (HPV-16) epitopes fused to the sequence of recombinant Potato virus A coat protein (ACP). Epitope derived from minor capsid protein L2 was expressed as an N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was fused to its C-terminus. The 885 bp construct (named L2ACPE7) was cloned into Potato virus X (PVX) based vector pGR106. In our experiments we compared two different means of agrobacterium-mediated infection in N. benthamiana plants; agroinfection using either hypodermic syringe or vacuum infiltration. Furthemore, we describe the possibilityof L2ACPE7 expression in an edible plant B. rapa, cv. Rapa. The presence and expression of resulting L2ACPE7 in B.rapa infected plants were confirmed by Immuno-Capture Reverse Trascription PCR (ICRT PCR), Plate-Trapped Antigen ELISA (PTA-

Czech name

Transient expression of fusion gene coding for the HPV-16 epitopes fused to the sequence of potyvirus coat protein using different means of inoculation of Nicotiana benthamiana and Brassica rapa, cv. Rapa plants

Czech description

We describe the effect of the different means of inoculation of Nicotina benthamiana plants on the amount of transiently expressed fussion gene coding for the Human papillomavirus type 16 (HPV-16) epitopes fused to the sequence of recombinant Potato virus A coat protein (ACP). Epitope derived from minor capsid protein L2 was expressed as an N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was fused to its C-terminus. The 885 bp construct (named L2ACPE7) was cloned into Potato virus X (PVX) based vector pGR106. In our experiments we compared two different means of agrobacterium-mediated infection in N. benthamiana plants; agroinfection using either hypodermic syringe or vacuum infiltration. Furthemore, we describe the possibilityof L2ACPE7 expression in an edible plant B. rapa, cv. Rapa. The presence and expression of resulting L2ACPE7 in B.rapa infected plants were confirmed by Immuno-Capture Reverse Trascription PCR (ICRT PCR), Plate-Trapped Antigen ELISA (PTA-

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

<a href="/en/project/GA521%2F06%2F0973" target="_blank" >GA521/06/0973: Expression of papillomavirus antigenic epitopes in plants using plant viral vector</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell Tissue and Organ Culture

ISSN

0167-6857

e-ISSN

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Volume of the periodical

19

Issue of the periodical within the volume

94

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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