The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10123306" target="_blank" >RIV/00216208:11310/12:10123306 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/12:00381055 RIV/60460709:41210/12:57557
Result on the web
<a href="http://dx.doi.org/10.1007/s10535-012-0130-2" target="_blank" >http://dx.doi.org/10.1007/s10535-012-0130-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10535-012-0130-2" target="_blank" >10.1007/s10535-012-0130-2</a>

Result language
angličtina
Original language name
The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties
Original language description
The Potato virus X (PVX)-based vector was used for the construction of N- and C-terminally modified PVX coat protein (XCP) chimeras. N-terminal XCP modifications do not influence the viral life cycle, whereas the simple XCP C-terminal fusion impedes theviral replication. We designed several C-terminally modified XCP chimeras and tested their viabilities in various Nicotiana benthamiana genotypes. Our results showed the negative impact of 3''-terminal modification of XCP on the chimera''s life cycle. Toensure chimeric constructs stability, the second copy of the last 60 nucleotides of XCP followed by the 3''-untranslated region (UTR) was added downstream of the recombinant sequence. Simultaneously, the first copy of the last 60 nucleotides of XCP wasmutated in order to prevent recombination between the two identical sequences. The movement protein of Tobacco mosaic virus expressed in transgenic N. benthamiana plants positively affected the cell-to-cell spread of C-terminally modified
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
—

Project
<a href="/en/project/GA521%2F09%2F1525" target="_blank" >GA521/09/1525: Biosafe plant virus expression system for transient production of human papillomavirus oncoproteins and their use for therapeutic vaccine development</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)