Identification and subcellular localization of molecular complexes of Gq/11? protein in HEK293 cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10123868" target="_blank" >RIV/00216208:11310/12:10123868 - isvavai.cz</a>
Result on the web
<a href="http://abbs.oxfordjournals.org/" target="_blank" >http://abbs.oxfordjournals.org/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/abbs/gms050" target="_blank" >10.1093/abbs/gms050</a>
Alternative languages
Result language
angličtina
Original language name
Identification and subcellular localization of molecular complexes of Gq/11? protein in HEK293 cells
Original language description
Heterotrimeric G-proteins localized in the plasma membrane convey the signals from G-protein-coupled receptors (GPCRs) to different effectors. At least some types of G-protein ? subunits have been shown to be partly released from plasma membranes and tomove into the cytosol after receptor activation by the agonists. However, the mechanism underlying subcellular redistribution of trimeric G-proteins is not well understood and no definitive conclusions have been reached regarding the translocation of G?subunits between membranes and cytosol. Here we used subcellular fractionation and clear-native polyacrylamide gel electrophoresis to identify molecular complexes of G(q/11)? protein and to determine their localization in isolated fractions and stabilityin na?ve and thyrotropin-releasing hormone (TRH)-treated HEK293 cells expressing high levels of TRH receptor and G(11)? protein. We identified two high-molecular-weight complexes of 300 and 140 kDa in size comprising the G(q/11) protein,
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Biochimica et Biophysica Sinica
ISSN
1672-9145
e-ISSN
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Volume of the periodical
44
Issue of the periodical within the volume
8
Country of publishing house
CN - CHINA
Number of pages
9
Pages from-to
641-649
UT code for WoS article
000306922800003
EID of the result in the Scopus database
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