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The time-course of agonist-induced solubilization of trimeric Gq?/G11? proteins resolved by two-dimensional electrophoresis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F08%3A00320703" target="_blank" >RIV/67985823:_____/08:00320703 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    The time-course of agonist-induced solubilization of trimeric Gq?/G11? proteins resolved by two-dimensional electrophoresis

  • Original language description

    In this study, we have used 2D electrophoresis for more defined resolution of Galpha subunits of G(q)alpha/G(11)alpha family from particulate membrane fraction and soluble (cytosol) cell fraction isolated as 250,000 x g supernatant and followed the timecourse of solubilization effect. Prolonged agonist stimulation results in specific transfer of activated Galpha subunits of G(q)alpha/G(11)alpha family from membrane fraction to cytosol. Solubilization of this class of Galpha proteins was observed afterprolonged agonist stimulation only, induced by ultra high concentration of hormone and in cells expressing a large number of GPCRs, therefore rather indicate tight/persisting binding of G(q)alpha/G(11)alpha proteins to the membrane

  • Czech name

    Časový průběh solubilizace trimerních Gq?/G11? proteinů indukované agonistou

  • Czech description

    V této studii jsme použili 2D elektroforézu pro lepší rozlišení Galfa podjednotek třídy G(q)alfa/G(11)alfa z membránové a cytosolární buněčné frakce izolované jako supernatant při 250000 x g a sledovali jsme časový průběh sloubilizace těchto proteinů. Dlouhodobá stimulace agonistou vedla k přesunu aktivovaných G(q)alfa/G(11)alfa proteinů z membránové frakce do cytosolu. Solubilizace této třídy Galfa proteinů byla pozorována pouze po dlouhodobé stimulaci agonistou ve vysoké koncentraci a v bňkách exprimujících vysoké hladiny GPCR, což ukazuje na pevnou vazbu G(q)alfa/G(11)alfa proteinů k membráně

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Physiological Research

  • ISSN

    0862-8408

  • e-ISSN

  • Volume of the periodical

    57

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    9

  • Pages from-to

  • UT code for WoS article

    257270700006

  • EID of the result in the Scopus database