Efficient expression of Human papillomavirus 16 E7 oncoprotein fused to C-terminus of Tobacco mosaic virus (TMV) coat protein using molecular chaperones in Escherichia coli
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10125286" target="_blank" >RIV/00216208:11310/12:10125286 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/12:00380787
Result on the web
<a href="http://dx.doi.org/10.1016/j.pep.2012.07.008" target="_blank" >http://dx.doi.org/10.1016/j.pep.2012.07.008</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.pep.2012.07.008" target="_blank" >10.1016/j.pep.2012.07.008</a>
Alternative languages
Result language
angličtina
Original language name
Efficient expression of Human papillomavirus 16 E7 oncoprotein fused to C-terminus of Tobacco mosaic virus (TMV) coat protein using molecular chaperones in Escherichia coli
Original language description
The Human papillomavirus 16 (HPV16)E7 oncoprotein is a promising candidate for development of anticancer therapeutic vaccine. We have prepared the expression construct carrying mutagenized E7 oncoprotein fused to the C-terminus of Tobacco mosaic virus (TMV) coat protein via 15 amino acids beta-sheet linker. The fusion protein was expressed in Escherichia coli MC 1061 cells. We have obtained high level expression, but most of the protein remained in insoluble inclusion bodies. To increase the ratio of soluble protein various molecular chaperones (TF, DnaK-Dnai-GrpE, GroEL-GroES) were used. The immunological reactivity of expressed recombinant protein was evaluated with anti-E7 and anti-TMV antibodies. The distribution of expressed product during ultracentrifugation on sucrose gradient was studied. (c) 2012 Elsevier Inc. All rights reserved.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Protein Expression and Purification
ISSN
1046-5928
e-ISSN
—
Volume of the periodical
85
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
152-157
UT code for WoS article
000308630400021
EID of the result in the Scopus database
—