Automated assay of the potency of natural antioxidants using pipetting robot and spectrophotometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10126275" target="_blank" >RIV/00216208:11310/12:10126275 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G44__/12:43874623 RIV/00216208:11130/12:8087 RIV/00064203:_____/12:8087
Result on the web
<a href="http://dx.doi.org/10.2478/v10136-012-0006-y" target="_blank" >http://dx.doi.org/10.2478/v10136-012-0006-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/v10136-012-0006-y" target="_blank" >10.2478/v10136-012-0006-y</a>

Result language
angličtina
Original language name
Automated assay of the potency of natural antioxidants using pipetting robot and spectrophotometry
Original language description
In the food industry, in the process of creating new agricultural plant products, and in the testing of anti-cancer drugs there is often a need to assay multiple samples of low molecular weight antioxidants, plant samples and foods rich in antioxidants,with minimal additional costs and low degrees of uncertainty. With these demands in mind, we decided to study the fully automated assay of antioxidants using not only automated sample measurements but also automated processing of samples and applicationof reagents. The automated pipetting system epMotion 5075 and the automated spectrophotometer BS 400 were chosen for the assay purposes. Five methods were introduced for the automation: 2-diphenyl-1-picrylhydrazyl (DPPH) test, ferric reducing antioxidantpower (FRAP) method, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) based test, N,N-dimethyl-1,4-diaminobenzene (DMPD) based test and the free radicals method. Samples containing one of the four antioxidants (standard rutin
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Project
<a href="/en/project/GAP301%2F10%2F0356" target="_blank" >GAP301/10/0356: Study of contribution of different DNA-damaging mechanisms to toxicity of cytostatics to human chemosensitive and chemoresistant neuroblastomas</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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