Automated assay of natural antioxidants potency using pipetting robot and spectrophotometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F12%3A00187243" target="_blank" >RIV/62156489:43210/12:00187243 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.2478/v10136-012-0006-y" target="_blank" >http://dx.doi.org/10.2478/v10136-012-0006-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/v10136-012-0006-y" target="_blank" >10.2478/v10136-012-0006-y</a>

Result language

angličtina

Original language name

Automated assay of natural antioxidants potency using pipetting robot and spectrophotometry

Original language description

We decided to study the fully automated assay of antioxidants using not only automated sample measurements but also automated processing of samples and application of reagents. The automated pipetting system epMotion 5075 and the automated spectrophotometer BS 400 were chosen for the assay purposes. Five methods were introduced for the automation: 2-diphenyl-1-picrylhydrazyl (DPPH) test, ferric reducing antioxidant power (FRAP) method, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) based test, N,N-dimethyl-1,4-diaminobenzene (DMPD) based test and the free radicals method. Samples containing one of the four antioxidants (standard rutin, quercitrin, ferulic and gallic acid) in a range 1--1000 ug/ml were used throughout. All of the tested methods were found suitable for implementation in an automated assay. However, some of them, such as the ABTS test failed to assay all tested antioxidants. The coefficients of determination were also unequal. FRAP methods provided the most

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of applied biomedicine

ISSN

1214-021X

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

13

Pages from-to

155-167

UT code for WoS article

304188400003

EID of the result in the Scopus database

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