(Strept)avidin-biotin interactions at amalgam electrodes covered by thiol monolayer
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10126595" target="_blank" >RIV/00216208:11310/12:10126595 - isvavai.cz</a>
Alternative codes found
RIV/61388955:_____/12:00377444
Result on the web
<a href="http://www.bestservis.eu/upload/file/Sbornik_metody12.pdf" target="_blank" >http://www.bestservis.eu/upload/file/Sbornik_metody12.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
(Strept)avidin-biotin interactions at amalgam electrodes covered by thiol monolayer
Original language description
Carboxylic group of 11-mercaptoundecanoic acid (MUA) can be used to creat a peptide bond with species containing amino group, e.g., peptides, and proteins. By the help of EDC-NHS technology, streptavidin or avidin was covalently bonded with MUA-monolayerat a silver solid amalgam electrode. Such prepared electrode was used for detecting biotin and biotinylated albumin in the supporting electrolyte (0.15 M NaCl, 0.05 M TRIS, pH 7.0). Electrochemical impendance spectroscopy was performed for the biosensorresponse monitored by impedance spectroscopy. Binding of biotin or biotinylated albumin with (strept)avidin a change in the resistance of the sensor in the concentration range of 0.5-20 ?g mL-1. Electrochemical regeneration of the amalgam electrode permits simply to renew its surface and to create the new biosensor.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CG - Electrochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
XXXII. Moderní Elektrochemické Metody Jetřichovice 21. - 25. května 2012 : Sborník přednášek mezinárodní odborné konference
ISBN
978-80-905221-0-7
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
155-158
Publisher name
Srsenová Lenka - BEST servis Ústí nad Labem
Place of publication
Ústí nad Labem
Event location
Jetřichovice, Czech Republic
Event date
May 21, 2012
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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