Mapping of interaction between cytochrome P450 2B4 and cytochrome b5: the first evidence of two mutual orientations

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10128683" target="_blank" >RIV/00216208:11310/12:10128683 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/12:00391101

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Mapping of interaction between cytochrome P450 2B4 and cytochrome b5: the first evidence of two mutual orientations

Original language description

The cytochrome P450 (P450) and cytochrome b5 are membrane hemoproteins composing together with flavoprotein NADPH:P450 reductase a mixed function oxidase (MFO) system. The knowledge of the interaction between P450 and its redox partners within a MFO system is fundamental to understand P450 reaction mechanism, an electron transport from its redox partner and also detoxification of xenobiotics and/or metabolism of endogenous substrates with all positive or negative aspects for organisms. The cross-linkingreaction was accomplished in an equimolar catalytic complex of P450 2B4:cytochrome b5 and the covalent hetero-dimers detected on SDS-PAGE electrophoresis were analyzed (after in gel trypsin digestion) using LC-HRMS to identify cross-linked amino-acid residues. The computed in silico models of P450 2B4:cytochrome b5 complex using amino-acids participating in cross-links (Asp134, Lys139, Glu424 and Glu439 located on a proximal surface of P450 2B4) suggest interpretation that two different

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GAP207%2F12%2F0627" target="_blank" >GAP207/12/0627: MAMMALIAN MICROSOMAL CYTOCHROME P450 INTERACTION WITH REDOX PARTNERS - TOPOLOGY AND STRUCTURE-FUNCTION RELATIONSHIPS</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Neuroendocrinology Letters

ISSN

0172-780X

e-ISSN

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Volume of the periodical

33

Issue of the periodical within the volume

Suppl 3

Country of publishing house

SE - SWEDEN

Number of pages

7

Pages from-to

41-47

UT code for WoS article

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EID of the result in the Scopus database

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