Quantification of interactions between cytochrome P450 2B4 and cytochrome b5 in a functional membrane complex

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10286787" target="_blank" >RIV/00216208:11310/14:10286787 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/14:00507239

Result on the web

<a href="http://www.nel.edu/archive_issues/o/35_s2/35_s2_Jecmen_114-122.pdf" target="_blank" >http://www.nel.edu/archive_issues/o/35_s2/35_s2_Jecmen_114-122.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Quantification of interactions between cytochrome P450 2B4 and cytochrome b5 in a functional membrane complex

Original language description

OBJECTIVES: The mammalian mixed function oxidase (MFO) system participates in hydroxylation of many hydrophobic endogenous compounds as well as xenobiotics such as drugs and carcinogens. This biotransformation system, located in a membrane of endoplasmicreticulum, consists of cytochrome P-450 (P450), NADPH:P450 oxidoreductase and a facultative component, cytochrome b5. The knowledge of the interactions among the individual components of the MFO system is essential to understand the relationships between the structure and function of this system that finally dictate a qualitative and quantitative pattern of produced metabolites (e.g. detoxified xenobiotics and/or activated carcinogens). To elucidate the quantitative aspects of the interactions within the MFO system we acquired the photo-initiated cross-linking approach. RESULTS: The successfully produced cytochrome b5 nanoprobe (with confirmed pMet incorporation by mass spectrometry) stimulates the catalytical activity of P450 2B4 when

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Neuroendocrinology Letters

ISSN

0172-780X

e-ISSN

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Volume of the periodical

35

Issue of the periodical within the volume

Suppl.2

Country of publishing house

SE - SWEDEN

Number of pages

9

Pages from-to

114-122

UT code for WoS article

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EID of the result in the Scopus database

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