Proteins implicated in the increase of adhesivity induced by suberoylanilide hydroxamic acid in leukemic cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10130575" target="_blank" >RIV/00216208:11310/12:10130575 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/12:00390148 RIV/00023736:_____/12:00010315
Result on the web
<a href="http://dx.doi.org/10.1016/j.jprot.2012.09.014" target="_blank" >http://dx.doi.org/10.1016/j.jprot.2012.09.014</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jprot.2012.09.014" target="_blank" >10.1016/j.jprot.2012.09.014</a>
Alternative languages
Result language
angličtina
Original language name
Proteins implicated in the increase of adhesivity induced by suberoylanilide hydroxamic acid in leukemic cells
Original language description
We have previously shown that suberoylanilide hydroxamic acid (SAHA) treatment increases the adhesivity of leukemic cells to fibronectin at clinically relevant concentrations. Now, we present the results of the proteomic analysis of SAHA effects on leukemic cell lines using 2-DE and ProteomLab PF2D system. Histone acetylation at all studied acetylation sites reached the maximal level after 5 to 10 h of SAHA treatment No difference in histone acetylation between subtoxic and toxic SAHA doses was observed. SAHA treatment induced cofilin phosphorylation at Ser3, an increase in vimentin and paxillin expression and a decrease in stathmin expression as confirmed by western-blotting and immunofluorescence microscopy. The interaction of cofilin with 14-3-3 epsilon was documented using both Duolink system and coimmunoprecipitation. However, this interaction was independent of cofilin Ser3 phosphorylation and the amount of 14-3-3-epsilon-bound cofilin did not rise following SAHA treatment SARA-i
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EA - Morphology and cytology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA301%2F09%2F1026" target="_blank" >GA301/09/1026: Proteomic analysis of suberoylanilide hydroxamic acid (SAHA) effects on human leukemic cells</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Proteomics
ISSN
1874-3919
e-ISSN
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Volume of the periodical
77
Issue of the periodical within the volume
25. září
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
17
Pages from-to
406-422
UT code for WoS article
000313535900032
EID of the result in the Scopus database
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