Proteome and metabolome profiling of cytokinin action in Arabidopsis identifies distinct but also similar responses to cytokinin down- and up-regulation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10133672" target="_blank" >RIV/00216208:11310/13:10133672 - isvavai.cz</a>

Result on the web

<a href="http://jxb.oxfordjournals.org/content/early/2013/09/20/jxb.ert227.full" target="_blank" >http://jxb.oxfordjournals.org/content/early/2013/09/20/jxb.ert227.full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/ert227" target="_blank" >10.1093/jxb/ert227</a>

Result language

angličtina

Original language name

Proteome and metabolome profiling of cytokinin action in Arabidopsis identifies distinct but also similar responses to cytokinin down- and up-regulation

Original language description

In plants, numerous developmental processes are controlled by cytokinin (CK) levels and their ratios to levels of other hormones. While molecular mechanisms underlying regulatory roles of CKs have been intensely researched, proteomic and metabolomic responses to CK deficiency are unknown. Transgenic Arabidopsis seedlings carrying inducible barley cytokinin oxidase/dehydrogenase (CaMV35S>GR>HvCKX2) and Agrobacterial isopentenyltransferase (CaMV35S>GR>ipt) constructs were profiled to elucidate proteome- and metabolome-wide responses to down- and up-regulation of CK levels, respectively. Proteome profiling identified >1100 proteins, 155 of which responded to HvCKX2 and/or ipt activation, mostly involved in growth, development and/or hormone and light signaling. The metabolome profiling covered 79 metabolites, 33 of which responded to HvCKX2 and/or ipt activation, mostly amino acids, carbohydrates and organic acids. Comparison of the datasets obtained from activated CaMV35S>GR>HvCKX2 and C

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

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Volume of the periodical

64

Issue of the periodical within the volume

14

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

4193-4206

UT code for WoS article

000326724700008

EID of the result in the Scopus database

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