Proteome and metabolome profiling of cytokinin action in Arabidopsis identifying both distinct and similar responses to cytokinin down- and up-regulation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33147817" target="_blank" >RIV/61989592:15310/13:33147817 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/13:00422255 RIV/62156489:43210/13:00205173 RIV/61389030:_____/13:00422255
Result on the web
<a href="http://dx.doi.org/10.1093/jxb/ert227" target="_blank" >http://dx.doi.org/10.1093/jxb/ert227</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jxb/ert227" target="_blank" >10.1093/jxb/ert227</a>

Result language
angličtina
Original language name
Proteome and metabolome profiling of cytokinin action in Arabidopsis identifying both distinct and similar responses to cytokinin down- and up-regulation
Original language description
In plants, numerous developmental processes are controlled by cytokinin (CK) levels and their ratios to levels of other hormones. While molecular mechanisms underlying the regulatory roles of CKs have been intensely researched, proteomic and metabolomicresponses to CK deficiency are unknown. Transgenic Arabidopsis seedlings carrying inducible barley cytokinin oxidase/dehydrogenase (CaMV35S}GR}HvCKX2) and agrobacterial isopentenyl transferase (CaMV35S}GR}ipt) constructs were profiled to elucidate proteome- and metabolome-wide responses to down- and up-regulation of CK levels, respectively. Proteome profiling identified }1100 proteins, 155 of which responded to HvCKX2 and/or ipt activation, mostly involved in growth, development, and/or hormone and light signalling. The metabolome profiling covered 79 metabolites, 33 of which responded to HvCKX2 and/or ipt activation, mostly aminoacids, carbohydrates, and organic acids. Comparison of the data sets obtained from activated CaMV35S}GR}HvCK
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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