Metastasis of aggressive amoeboid sarcoma cells is dependent on Rho/ROCK/MLC signaling

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10189096" target="_blank" >RIV/00216208:11310/13:10189096 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/13:00423218

Result on the web

<a href="http://dx.doi.org/10.1186/1478-811X-11-51" target="_blank" >http://dx.doi.org/10.1186/1478-811X-11-51</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1478-811X-11-51" target="_blank" >10.1186/1478-811X-11-51</a>

Result language

angličtina

Original language name

Metastasis of aggressive amoeboid sarcoma cells is dependent on Rho/ROCK/MLC signaling

Original language description

Background: Although there is extensive evidence for the amoeboid invasiveness of cancer cells in vitro, much less is known about the role of amoeboid invasiveness in metastasis and the importance of Rho/ROCK/MLC signaling in this process. Results: We analyzed the dependence of amoeboid invasiveness of rat and chicken sarcoma cells and the metastatic activity of chicken cells on individual elements of the Rho/ROCK/MLC pathway. In both animal models, inhibition of Rho, ROCK or MLC resulted in greatly decreased cell invasiveness in vitro, while inhibition of extracellular proteases using a broad spectrum inhibitor did not have a significant effect. The inhibition of both Rho activity and MLC phosphorylation by dominant negative mutants led to a decreasedcapability of chicken sarcoma cells to metastasize. Moreover, the overexpression of RhoA in non-metastatic chicken cells resulted in the rescue of both invasiveness and metastatic capability. Rho and ROCK, unlike MLC, appeared to be dire

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/LC06061" target="_blank" >LC06061: Center of Cell Invasiveness in Embryonic Development and Tumour Metastases</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cell Communication and Signaling

ISSN

1478-811X

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

July 2013

Country of publishing house

IL - THE STATE OF ISRAEL

Number of pages

13

Pages from-to

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UT code for WoS article

000322740600001

EID of the result in the Scopus database

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