Susceptibility of clinical isolates of Leishmania aethiopica to miltefosine, paromomycin, amphotericin B and sodium stibogluconate using amastigote-macrophage in vitro model

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10190420" target="_blank" >RIV/00216208:11310/13:10190420 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.exppara.2013.01.022" target="_blank" >http://dx.doi.org/10.1016/j.exppara.2013.01.022</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.exppara.2013.01.022" target="_blank" >10.1016/j.exppara.2013.01.022</a>

Result language
angličtina
Original language name
Susceptibility of clinical isolates of Leishmania aethiopica to miltefosine, paromomycin, amphotericin B and sodium stibogluconate using amastigote-macrophage in vitro model
Original language description
Cutaneous Leishmaniasis (CL) caused by Leishmania aethiopica is a public health and social problem with a sequel of severe and mutilating skin lesions. It is manifested in three forms: localized CL (LCL), mucosal CL (MCL) and diffuse CL (DCL). Unresponsiveness to sodium stibogluconate (Sb-V) is common in Ethiopian CL patients. Using the amastigote-macrophage in vitro model the susceptibility of 24 clinical isolates of L. aethiopica derived from untreated patients was investigated. Eight strains of LCL,9 of MCL, and 7 of DCL patients together with a reference strain (MHOM/ET/82/117/82) were tested against four antileishmanial drugs: amphotericin B, miltefosine, Sb-V and paromomycin. In the same order of drugs, IC50 (mu g/ml +/- SD) values for the 24 strains tested were 0.16 +/- 0.18, 5.88 +/- 4.79, 10.23 +/- 8.12, and 13.63 +/- 18.74. The susceptibility threshold of isolates originating from the 3 categories of patients to all 4 drugs was not different (p > 0.05). Maximal efficacy was
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FN - Epidemiology, infection diseases and clinical immunology
OECD FORD branch
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Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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