Molecular rheometry: direct determination of viscosity in L-o and L-d lipid phases via fluorescence lifetime imaging
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10192273" target="_blank" >RIV/00216208:11310/13:10192273 - isvavai.cz</a>
Alternative codes found
RIV/61388955:_____/13:00394499
Result on the web
<a href="http://dx.doi.org/10.1039/c3cp51953h" target="_blank" >http://dx.doi.org/10.1039/c3cp51953h</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c3cp51953h" target="_blank" >10.1039/c3cp51953h</a>
Alternative languages
Result language
angličtina
Original language name
Molecular rheometry: direct determination of viscosity in L-o and L-d lipid phases via fluorescence lifetime imaging
Original language description
Understanding of cellular regulatory pathways that involve lipid membranes requires the detailed knowledge of their physical state and structure. However, mapping the viscosity and diffusion in the membranes of complex composition is currently a non-trivial technical challenge. We report fluorescence lifetime spectroscopy and imaging (FLIM) of a meso-substituted BODIPY molecular rotor localised in the leaflet of model membranes of various lipid compositions. We prepare large and giant unilamellar vesicles (LUVs and GUVs) containing phosphatidylcholine (PC) lipids and demonstrate that recording the fluorescence lifetime of the rotor allows us to directly detect the viscosity of the membrane leaflet and to monitor the influence of cholesterol on membraneviscosity in binary and ternary lipid mixtures. In phase-separated 1,2-dioleoyl-sn-glycero-3-phosphocholine-cholesterol-sphingomyelin GUVs we visualise individual liquid ordered (L-o) and liquid disordered (L-d) domains using FLIM and as
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physical Chemistry Chemical Physics
ISSN
1463-9076
e-ISSN
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Volume of the periodical
15
Issue of the periodical within the volume
36
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
14986-14993
UT code for WoS article
000323520600019
EID of the result in the Scopus database
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