Time-Resolved Laurdan Fluorescence Reveals Insights into Membrane Viscosity and Hydration Levels
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10388460" target="_blank" >RIV/00216208:11310/18:10388460 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.bpj.2018.08.041" target="_blank" >https://doi.org/10.1016/j.bpj.2018.08.041</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bpj.2018.08.041" target="_blank" >10.1016/j.bpj.2018.08.041</a>
Alternative languages
Result language
angličtina
Original language name
Time-Resolved Laurdan Fluorescence Reveals Insights into Membrane Viscosity and Hydration Levels
Original language description
Membrane viscosity and hydration levels characterize the biophysical properties of biological membranes and are reflected in the rate and extent of solvent relaxation, respectively, of environmentally sensitive fluorophores such as Laurdan. Here, we first developed a method for a time-resolved general polarization (GP) analysis with fluorescence-lifetime imaging microscopy that captures both the extent and rate of Laurdan solvent relaxation. We then conducted time-resolved GP measurements with Laurdan-stained model membranes and cell membranes. These measurements revealed that cholesterol levels in lipid vesicles altered membrane hydration and viscosity, whereas curvature had little effect on either parameter. We also applied the method to the plasma membrane of live cells using a supercritical angle fluorescence objective, to our knowledge the first time fluorescence-lifetime imaging microscopy images were generated with supercritical angle fluorescence. Here, we found that local variations in membrane cholesterol most likely account for the heterogeneity of Laurdan lifetime in plasma membrane. In conclusion, time-resolved GP measurements provide additional insights into the biophysical properties of membranes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
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Continuities
O - Projekt operacniho programu
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biophysical Journal
ISSN
0006-3495
e-ISSN
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Volume of the periodical
115
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
1498-1508
UT code for WoS article
000447398300009
EID of the result in the Scopus database
2-s2.0-85054006004