DNA-barcoding: A case study in the diatom genus Frustulia (Bacillariophyceae)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10194130" target="_blank" >RIV/00216208:11310/13:10194130 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
DNA-barcoding: A case study in the diatom genus Frustulia (Bacillariophyceae)
Original language description
Frustulia was used as a model genus to test four candidate markers (D1-D2 LSU rDNA, V4-SSU rDNA, rbcL-3 P, and COI-5 P) for DNA barcoding of diatoms. The Frustulia strains included in the study were primarily isolated from Europe and New Zealand. In agreement with previous reports, the results suggest that a dual-locus barcode comprising a partial sequence of the large ribosomal subunit (LSU) and a partial sequence of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) is the best option for DNA barcoding of diatoms. Unfortunately, this approach is limited by the lack of a distinct barcode gap for these markers. Overall, the results of this study suggest that Frustulia species cannot currently be resolved without the use of additional non-molecular evidence. Effective use of DNA barcoding will require a better understanding of the mechanisms that generate and maintain genetic diversity in diatoms.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GP13-07210P" target="_blank" >GP13-07210P: Diversity within the Frustulia rhomboides species complex (Bacillariophyceae): a multidisciplinary approach.</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nova Hedwigia
ISSN
0029-5035
e-ISSN
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Volume of the periodical
Neuveden
Issue of the periodical within the volume
142
Country of publishing house
DE - GERMANY
Number of pages
16
Pages from-to
147-162
UT code for WoS article
000325381600013
EID of the result in the Scopus database
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