PacBio sequencing of Glomeromycota rDNA: a novel amplicon covering all widely used ribosomal barcoding regions and its applicability in taxonomy and ecology of arbuscular mycorrhizal fungi
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00546867" target="_blank" >RIV/61388971:_____/21:00546867 - isvavai.cz</a>
Alternative codes found
RIV/67985939:_____/21:00545683 RIV/00216208:11310/21:10432693
Result on the web
<a href="https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.17372" target="_blank" >https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.17372</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/nph.17372" target="_blank" >10.1111/nph.17372</a>
Alternative languages
Result language
angličtina
Original language name
PacBio sequencing of Glomeromycota rDNA: a novel amplicon covering all widely used ribosomal barcoding regions and its applicability in taxonomy and ecology of arbuscular mycorrhizal fungi
Original language description
There is no consensus barcoding region for determination of arbuscular mycorrhizal fungal (AMF) taxa. To overcome this obstacle, we have developed an approach to sequence an AMF marker within the ribosome-encoding operon (rDNA) that covers all three widely applied variable molecular markers.nUsing a nested PCR approach specific to AMF, we amplified a part (c. 2.5 kb) of the rDNA spanning the majority of the small subunit rRNA (SSU) gene, the complete internal transcribed spacer (ITS) region and a part of the large subunit (LSU) rRNA gene. The PCR products were sequenced on the PacBio platform utilizing Single Molecule Real Time (SMRT) sequencing.nEmploying this method for selected environmental DNA samples, we were able to describe complex AMF communities consisting of various glomeromycotan lineages.nWe demonstrate the applicability of this new 2.5 kb approach to provide robust phylogenetic assignment of AMF lineages without known sequences from pure cultures and to consolidate information about AMF taxon distributions coming from three widely used barcoding regions into one integrative dataset.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA17-03921S" target="_blank" >GA17-03921S: Sequence-based phylogeny of Glomeromycota: from virtual taxa to phylospecies and global ecological and biogeographical patterns</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
New Phytologist
ISSN
0028-646X
e-ISSN
1469-8137
Volume of the periodical
231
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
490-499
UT code for WoS article
000645953600001
EID of the result in the Scopus database
2-s2.0-85105234417