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De Novo Biosynthesis of Sexual Pheromone in the Labial Gland of Bumblebee Males

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10209509" target="_blank" >RIV/00216208:11310/13:10209509 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388963:_____/13:00391726

  • Result on the web

    <a href="http://dx.doi.org/10.1002/cbic.201200684" target="_blank" >http://dx.doi.org/10.1002/cbic.201200684</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/cbic.201200684" target="_blank" >10.1002/cbic.201200684</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    De Novo Biosynthesis of Sexual Pheromone in the Labial Gland of Bumblebee Males

  • Original language description

    De novo biosynthesis of male sex pheromone from two bumblebee species (Bombus terrestris and Bombus lucorum) was studied by using in vitro incubations of labial glands (LGs) with radioactive [1,2-C-14] acetate and deuterated [D-3]acetate. The labeled substrate was incorporated into several types of compounds, such as terpenic alcohols, fatty acids, esters, and hydrocarbons. A similar incubation of [1,2-C-14] acetate with fat bodies (FB) led to the formation of fatty acids, triacylglycerols (TAG), and hydrocarbons. To support the results from in vitro incubations, PCR analysis of fatty acid synthase (FAS) transcripts in LG and FB was performed. Relative quantification of FAS transcription levels revealed that the abundance of mRNA from the FAS gene is afunction of the age of B. terrestris males. A comparison of the relative FAS mRNA gene transcription level in FB and LGs of B. terrestris and B. lucorum males proved that high biosynthetic activity takes place in the LGs of both species.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA203%2F09%2F1446" target="_blank" >GA203/09/1446: Study of Enzymes Involved in the Biosynthesis of Pheromones of Model Species of Bumblebees</a><br>

  • Continuities

    S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Chembiochem : a European journal of chemical biology

  • ISSN

    1439-4227

  • e-ISSN

  • Volume of the periodical

    14

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    11

  • Pages from-to

    361-371

  • UT code for WoS article

    000315491700012

  • EID of the result in the Scopus database