Exploring complex pheromone biosynthetic processes in the bumblebee male labial gland by RNA sequencing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00460681" target="_blank" >RIV/61388963:_____/16:00460681 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/imb.12221" target="_blank" >http://dx.doi.org/10.1111/imb.12221</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/imb.12221" target="_blank" >10.1111/imb.12221</a>

Result language

angličtina

Original language name

Exploring complex pheromone biosynthetic processes in the bumblebee male labial gland by RNA sequencing

Original language description

Male marking pheromones (MPs) are used by the majority of bumblebee species (Hymenoptera: Apidae), including a commercially important greenhouse pollinator, the buff-tailed bumblebee (Bombus terrestris), to attract conspecific females. MP biosynthetic processes in the cephalic part of the bumblebee male labial gland (LG) are of extraordinary complexity, involving enzymes of fatty acid and isoprenoid biosynthesis, which jointly produce more than 50 compounds. We employed a differential transcriptomic approach to identify candidate genes involved in MP biosynthesis by sequencing Bombus terrestris LG and fat body (FB) transcriptomes. We identified 12 454 abundantly expressed gene products (reads per kilobase of exon model per million mapped reads value > 1) that had significant hits in the GenBank nonredundant database. Of these, 876 were upregulated in the LG (> 4-fold difference). We identified more than 140 candidate genes potentially involved in MP biosynthesis, including esterases, fatty acid reductases, lipases, enzymes involved in limited fatty acid chain shortening, neuropeptide receptors and enzymes involved in biosynthesis of triacylglycerols, isoprenoids and fatty acids. For selected candidates, we confirmed their abundant expression in LG using quantitative real-time reverse transcription-PCR (qRT-PCR). Our study shows that the Bombus terrestris LG transcriptome reflects both fatty acid and isoprenoid MP biosynthetic processes and identifies rational gene targets for future studies to disentangle the molecular basis of MP biosynthesis. Additionally, LG and FB transcriptomes enrich the available transcriptomic resources for Bombus terrestris.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Insect Molecular Biology

ISSN

0962-1075

e-ISSN

—

Volume of the periodical

25

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

20

Pages from-to

295-314

UT code for WoS article

000375669800010

EID of the result in the Scopus database

2-s2.0-84959519716