Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10144018" target="_blank" >RIV/00216208:11310/14:10144018 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.2478/s11696-013-0422-9" target="_blank" >http://dx.doi.org/10.2478/s11696-013-0422-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/s11696-013-0422-9" target="_blank" >10.2478/s11696-013-0422-9</a>

Result language

angličtina

Original language name

Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography

Original language description

Fused silica capillary columns of the internal diameter of 320 mu m were packed with the Nucleosil C18 stationary phase of 5 mu m using the slurry packing method. The time of the bed compaction phase, packing pressure, and the use of ultrasound varied tostudy their influence on the column performance. Van Deemter curves were measured and separation impedance values were calculated in order to assess both separation efficiency and kinetic performance of the columns. Selected columns were tested again after nine months to evaluate the stability of their beds. Separation efficiencies of all columns were similar, but a major difference, caused by the use of ultrasound, was observed in the bed stability. Columns sonicated for 25 minutes during the bed compaction phase exhibited unchanged performance in the course of several months, while the performance of non-sonicated columns decreased. (C) 2013 Institute of Chemistry, Slovak Academy of Sciences

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemical Papers

ISSN

0366-6352

e-ISSN

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Volume of the periodical

68

Issue of the periodical within the volume

1

Country of publishing house

SK - SLOVAKIA

Number of pages

7

Pages from-to

22-28

UT code for WoS article

000324491200003

EID of the result in the Scopus database

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