Comparison of nano and conventional liquid chromatographic methods for the separation of ( )-catechin-ethyl-malvidin-3-glucoside diastereoisomers

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F16%3A33161047" target="_blank" >RIV/61989592:15310/16:33161047 - isvavai.cz</a>

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0021967315013941" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0021967315013941</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2015.09.074" target="_blank" >10.1016/j.chroma.2015.09.074</a>

Result language

angličtina

Original language name

Comparison of nano and conventional liquid chromatographic methods for the separation of ( )-catechin-ethyl-malvidin-3-glucoside diastereoisomers

Original language description

Nano-liquid chromatography and conventional HPLC were used for the separation of diastereomers of (+)-catechin-ethyl-malvidin-3-glucoside. Those bridged anthocyanin dyes were obtained by reaction of (+)-catechin with malvidin-3-glucoside in the presence of acetaldehyde. Both diastereomers were isolated with semipreparative chromatography and their structures were confirmed by nuclear magnetic resonance and mass spectrometry. In-laboratory prepared capillary columns packed with fully porous particles Chromosphere C18, dp = 3 mu m, core-shell particles Kinetex C18, dp = 2.6 mu m (100 mu m i.d.) and monolithic column Chromolith CapRod (100 mu m i.d.) were used for the separation of (+)-catechin, malvidin-3-glucoside and both diastereomers. Chromosphere C18 stationary phase provided the best chromatographic performance. Mobile phase containing water:acetonitrile (80:20) acidified with trifluoroacetic acid (0.1%, v/v/v) was used in an isocratic elution mode with a flow rate of 360 nL min(-1). Separation of studied compounds was achieved in less than 7 min under optimized conditions. The nano-liquid chromatographic method and a conventional HPLC one using the same fully porous particles (Chromosphere C18, 3 mu m, 100 mm x 4.6 mm) were compared providing higher separation efficiency with the first analytical method and similar selectivity. A better peak symmetry and higher resolution of the studied diastereomers was achieved by conventional chromatography. Nevertheless, nano-liquid chromatography appeared to be useful for the separation of complex anthocyanin dyes and can be utilized for their analysis in plant and food micro-samples. The developed method was used for analysis of red wine grape pomace

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography A: Symposium Volumes

ISSN

0021-9673

e-ISSN

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Volume of the periodical

1428

Issue of the periodical within the volume

JAN

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

126-133

UT code for WoS article

000368564000010

EID of the result in the Scopus database

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