Separation of structurally related anthocyanins by MEKC

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15410%2F03%3A00001320" target="_blank" >RIV/61989592:15410/03:00001320 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/03:00001320

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Separation of structurally related anthocyanins by MEKC

Original language description

A method based on micellar electrokinetic chromatography has been developed for the simultaneous separation of six anthocyanins (malvidin-3,5-diglucoside, molvidin-3-glucosicle, molvidin-3-galactoside, pelargonidin-3-glucoside, cyanidin-3,5-diglucoside,cyanidin-3-galactoside). Optimum selectivity was achieved in the buffer 30 mM phosphate + 400 mM borate-TRIS, pH = 7.0 supported with 50 mM sodium dodecylsulphate. High content of borate was essential mainly for the separation of diastereomeric pair malvidin-3-glucoside-malvidin-3-galactoside. The calibration dependencies exhibit good linearity in the ranges of concentration 10-100 mug mL(-1) for diglycosylated and 25-100 mug mL(-1) for monoglycosylated derivatives (R-2 = 0.9711-0.9989). The optimized method was applied to a sample of wine grape skin extract. Malvidin-3-glucoside was identified as main anthocyanin colorant in this sample.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GP203%2F02%2FD013" target="_blank" >GP203/02/D013: Analysis of anthocyanin dyes by high performance separation techniques hyphenated with mass spectrometry</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chromatographia

ISSN

0009-5893

e-ISSN

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Volume of the periodical

58

Issue of the periodical within the volume

5-6

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

5

Pages from-to

283-287

UT code for WoS article

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EID of the result in the Scopus database

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