Unbiased estimation of chloroplast number in mesophyll cells: advantage of a genuine three-dimensional approach

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10218317" target="_blank" >RIV/00216208:11310/14:10218317 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/14:00428273
Result on the web
<a href="http://dx.doi.org/10.1093/jxb/ert407" target="_blank" >http://dx.doi.org/10.1093/jxb/ert407</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jxb/ert407" target="_blank" >10.1093/jxb/ert407</a>

Result language
angličtina
Original language name
Unbiased estimation of chloroplast number in mesophyll cells: advantage of a genuine three-dimensional approach
Original language description
Chloroplast number per cell is a frequently examined quantitative anatomical parameter, often estimated by counting chloroplast profiles in two-dimensional (2D) sections of mesophyll cells. However, a mesophyll cell is a three-dimensional (3D) structureand this has to be taken into account when quantifying its internal structure. We compared 2D and 3D approaches to chloroplast counting from different points of view: (i) in practical measurements of mesophyll cells of Norway spruce needles, (ii) in a 3Dmodel of a mesophyll cell with chloroplasts, and (iii) using a theoretical analysis. We applied, for the first time, the stereological method of an optical disector based on counting chloroplasts in stacks of spruce needle optical cross-sections acquired by confocal laser-scanning microscopy. This estimate was compared with counting chloroplast profiles in 2D sections from the same stacks of sections. Comparing practical measurements of mesophyll cells, calculations performed in a 3D mo
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Project
<a href="/en/project/GAP501%2F10%2F0340" target="_blank" >GAP501/10/0340: Impact of CO2 enrichment and irradiance on structure and performance of forest tree photosynthetic apparatus at different hierarchical levels</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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