Unbiased estimation of Norway spruce (Picea abies L. Karst.) chloroplast structure: Heterogeneity within needle mesophyll under different irradiance and [CO 2 ]
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00504785" target="_blank" >RIV/67985823:_____/19:00504785 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/19:10394057
Result on the web
<a href="https://www.ias-iss.org/ojs/IAS/article/view/2005" target="_blank" >https://www.ias-iss.org/ojs/IAS/article/view/2005</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5566/ias.2005" target="_blank" >10.5566/ias.2005</a>
Alternative languages
Result language
angličtina
Original language name
Unbiased estimation of Norway spruce (Picea abies L. Karst.) chloroplast structure: Heterogeneity within needle mesophyll under different irradiance and [CO 2 ]
Original language description
The main objective of this study was to find out whether the selected chloroplast characteristics measured in the mesophyll layer nearest to the needle surface (i.e., the first mesophyll layer) could be representative for the whole needle cross section. Two chloroplast sampling approaches were applied on Norway spruce needles during the investigation of the effects of different levels of air CO2 concentration and irradiance: (i) sampling only from the first mesophyll layer, and (ii) systematic uniform random (SUR) sampling. The selected characteristics were: (i) chloroplast area, (ii) starch grain area, and (iii) starch areal density on median chloroplast cross sections, and (iv) chloroplast number per unit of needle volume. It was shown that the first mesophyll layer was not representative for estimating all evaluated characteristics except the chloroplast area. Sampling only there caused obtaining slightly biased results, while SUR sampling gave unbiased estimations at the cost of longer measuring time. The major effect of studied factors was in starch areal density and starch grain area, which were larger in sun needles in elevated CO2 concentration in comparison with sun needles in ambient CO2 concentration. In conclusion, it was demonstrated that the first layer of mesophyll is not always representative for the needle cross section. If technically feasible, SUR is recommended for analysis of chloroplast ultrastructure. The simplified sampling design can be applied, e.g., for comparisons of many different treatments. However, it should be combined with other approaches to characterize the chloroplast function and the results carefully considered and interpreted.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40102 - Forestry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Image Analysis and Stereology
ISSN
1580-3139
e-ISSN
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Volume of the periodical
38
Issue of the periodical within the volume
1
Country of publishing house
SI - SLOVENIA
Number of pages
10
Pages from-to
83-94
UT code for WoS article
000464195100009
EID of the result in the Scopus database
2-s2.0-85065480947