Opioid-Receptor (OR) Signaling Cascades in Rat Cerebral Cortex and Model Cell Lines: the Role of Plasma Membrane Structure
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10227230" target="_blank" >RIV/00216208:11310/14:10227230 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/14:00427645 RIV/61388955:_____/14:00427645
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Opioid-Receptor (OR) Signaling Cascades in Rat Cerebral Cortex and Model Cell Lines: the Role of Plasma Membrane Structure
Original language description
Proteomic analysis of plasma membrane fractions (PM) izolated from brain cortex of rats exposed to increasing doses of morphine (10-50 mg/kg) for 10 days indicated that the brain cortex of morphine-treated animals can not be regarded as being adapted tothis drug as significant up-regulation of proteins functionally related to oxidative stress and alternation of brain energy metabolism occurred. The number of delta-OR was increased 2x and their sensitivity to monovalent cations altered. Our studies of plasma membrane structure and function in context with desensitization of GPCR action were extended by data indicating participation of cholesterol-enriched membrane domains in agonist-specific internalization of delta-OR. In HEK293 cells stably expressing delta-OR-Gi1alfa fusion protein, depletion of PM cholesterol was associated with decrease (by two-orders of magnitude) in affinity /potency of G-protein-response to agonist stimulation; maximum response was unchanged. Hydrophobic interi
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physiological Research
ISSN
0862-8408
e-ISSN
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Volume of the periodical
63
Issue of the periodical within the volume
Suppl. 1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
12
Pages from-to
"S165"-"S176"
UT code for WoS article
000332073100014
EID of the result in the Scopus database
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