Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F14%3A00427791" target="_blank" >RIV/67985823:_____/14:00427791 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1186/1477-5956-12-11" target="_blank" >http://dx.doi.org/10.1186/1477-5956-12-11</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1477-5956-12-11" target="_blank" >10.1186/1477-5956-12-11</a>

Result language

angličtina

Original language name

Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine

Original language description

Proteomic analysis was performed in post-nuclear supernatant (PNS) and Percoll-purified membranes (PM) prepared from frontal brain cortex of rats exposed to increasing doses of morphine (10-50 mg/kg) for 10 days. In the PNS, the 10 up- or down-regulatedproteins exhibiting the largest morphine-induced change were selected, excised manually from the gel and identified by MALDI-TOF MS/MS. The identified proteins were of cytoplasmic, cell membrane, endoplasmic reticulum and mitochondrial origin and 9 of them were significantly increased. In PM, the 18 up- or down-regulated proteins were identified by LC-MS/MS and were of plasma membrane, myelin membrane, cytoplasmic and mitochondrial origin. The only member of GPCR-initiated signaling cascades identifiedin PM as altered by morphine was G? subunit. Surprisingly, the immunoblot analysis of the same PM indicated that the ?active, morphine-induced pool of G? subunits, represented just a minor fraction of the total signal of G? which was decr

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Proteome Science

ISSN

1477-5956

e-ISSN

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Volume of the periodical

12

Issue of the periodical within the volume

Feb 14

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

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UT code for WoS article

000332800900002

EID of the result in the Scopus database

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