Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals exposed to morphine and subsequently nurtured for 20 days in the absence of this drug

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F16%3A00461865" target="_blank" >RIV/67985823:_____/16:00461865 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.jprot.2016.02.019" target="_blank" >http://dx.doi.org/10.1016/j.jprot.2016.02.019</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jprot.2016.02.019" target="_blank" >10.1016/j.jprot.2016.02.019</a>

Result language
angličtina
Original language name
Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals exposed to morphine and subsequently nurtured for 20 days in the absence of this drug
Original language description
Proteomic analysis was performed in post-nuclear supernatant fraction (PNS) prepared from forebrain cortex of rats exposed to increasing doses of morphine (10–50 mg/kg) for 10 days and sacrificed 24 h (group + M10) or 20 days (group + M10/M20) after the last dose of morphine. PNS fraction was resolved by 2D-ELFO and stained by CBB. Analysis of the difference between (+ M10) and ( M10) samples of PNS by PDQuest accompanied by MALDI-TOF MS/MS indicated the significant change of 28 proteins. Importantly, the number of altered proteins was decreased to 14 after 20 days of nurturing animals in the absence of morphine. This new and important finding indicating the ability of mammalian organism to return to physiological norm after removal of the drug was verified by an independent methodology — gel-free & label-free quantification and normalization procedure denominated as MaxLFQ. The 113 proteins were identified as altered by morphine in (+ M10) samples when compared with ( M10) samples of PNS and this number was decreased to 19 after 20 days of nurturing the animals in the absence of this drug.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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