Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days, comparison with animals after 20 days of morphine withdrawal

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F20%3A00524280" target="_blank" >RIV/67985823:_____/20:00524280 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/20:10413205

Result on the web

<a href="https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0231721" target="_blank" >https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0231721</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0231721" target="_blank" >10.1371/journal.pone.0231721</a>

Result language

angličtina

Original language name

Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days, comparison with animals after 20 days of morphine withdrawal

Original language description

Opioid addiction is recognized as a chronic relapsing brain disease resulting from repeated exposure to opioid drugs. Cellular and molecular mechanisms underlying the ability of organism to return back to the physiological norm after cessation of drug supply are not fully understood. The aim of this work was to extend our previous studies of morphine-induced alteration of rat forebrain cortex protein composition to the hippocampus. Rats were exposed to morphine for 10 days and sacrificed 24 h (groups +M10 and -M10) or 20 days after the last dose of morphine (groups +M10/-M20 and -M10/-M20). The six altered proteins (>= 2-fold) were identified in group (+M10) when compared with group (-M10) by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE). The number of differentially expressed proteins was increased to thirteen after 20 days of the drug withdrawal. Noticeably, the altered level of alpha-synuclein, beta-synuclein, alpha-enolase and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was also determined in both (+/- M10) and (+/- M10/-M20) samples of hippocampus. Immunoblot analysis of 2D gels by specific antibodies oriented against alpha/beta-synucleins and GAPDH confirmed the data obtained by 2D-DIGE analysis. Label-free quantification identified nineteen differentially expressed proteins in group (+M10) when compared with group (-M10). After 20 days of morphine withdrawal (+/- M10/-M20), the number of altered proteins was increased to twenty. We conclude that the morphine-induced alteration of protein composition in rat hippocampus after cessation of drug supply proceeds in a different manner when compared with the forebrain cortex. In forebrain cortex, the total number of altered proteins was decreased after 20 days without morphine, whilst in hippocampus, it was increased.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS ONE

ISSN

1932-6203

e-ISSN

—

Volume of the periodical

15

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

25

Pages from-to

e0231721

UT code for WoS article

000529957800001

EID of the result in the Scopus database

2-s2.0-85083327650