Impact of three-month morphine withdrawal on rat brain cortex, hippocampus, striatum and cerebellum: proteomic and phosphoproteomic studies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F21%3A00542103" target="_blank" >RIV/67985823:_____/21:00542103 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/21:10431530

Result on the web

<a href="https://doi.org/10.1016/j.neuint.2021.104975" target="_blank" >https://doi.org/10.1016/j.neuint.2021.104975</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.neuint.2021.104975" target="_blank" >10.1016/j.neuint.2021.104975</a>

Result language

angličtina

Original language name

Impact of three-month morphine withdrawal on rat brain cortex, hippocampus, striatum and cerebellum: proteomic and phosphoproteomic studies

Original language description

Opioid addiction is characterized by compulsive drug seeking and taking behavior, which is thought to result from persistent neuroadaptations. However, there is a lack of information about the changes at both the cellular and molecular levels occurring after cessation of drug administration. The aim of our study was to determine alterations of both phosphoproteome and proteome in selected brain regions of the rats (brain cortex, hippocampus, striatum, and cerebellum) 3 months after cessation of 10-day morphine treatment. Phosphoproteome profiling was performed by Pro-Q® Diamond staining. The gel-based proteomic approach accompanied by label-free quantification (MaxLFQ) was used for characterization of proteome changes.The phosphoproteomic analysis revealed the largest change in the hippocampus (14), only few altered proteins were detected in the forebrain cortex (5), striatum (4), and cerebellum (3). The change of total protein composition, determined by 2D electrophoresis followed by LFQ analysis, identified 22 proteins with significantly altered expression levels in the forebrain cortex, 19 proteins in the hippocampus, 12 in the striatum and 10 in the cerebellum. The majority of altered proteins were functionally related to energy metabolism and cytoskeleton reorganization. As the most important change we regard down-regulation of 14-3-3 proteins in rat cortex and hippocampus.Our findings indicate that i) different parts of the brain respond in a distinct manner to the protracted morphine withdrawal, ii) characterize changes of protein composition in these brain parts, and iii) enlarge the scope of evidence for adaptability and distinct neuroplasticity proceeding in the brain of drug-addicted organism.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30105 - Physiology (including cytology)

Project

<a href="/en/project/GA19-03295S" target="_blank" >GA19-03295S: Consequences of sustained morphine treatment and withdrawal on the rat brain: proteomic and functional studies</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Neurochemistry International

ISSN

0197-0186

e-ISSN

1872-9754

Volume of the periodical

144

Issue of the periodical within the volume

Mar

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

104975

UT code for WoS article

000636076900001

EID of the result in the Scopus database

2-s2.0-85100027342