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Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10227341" target="_blank" >RIV/00216208:11310/14:10227341 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1371/journal.pntd.0002709" target="_blank" >http://dx.doi.org/10.1371/journal.pntd.0002709</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1371/journal.pntd.0002709" target="_blank" >10.1371/journal.pntd.0002709</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis

  • Original language description

    Two cDNA libraries were constructed from female salivary glands of P. orientalis from Addis Zemen and Melka Werer. Phylogenetic analysis were performed to evaluate differences between the P. orientalis colonies and to show the relationship with other sand fly species from the subgenus Larroussius. we investigated the humoral antigenicity and cross-reactivity of the salivary proteins and the activity of salivary apyrase and hyaluronidase. Our study expanded the knowledge of salivary gland compounds of sand fly species in the subgenus Larroussius. we showed that P. orientalis is closely related to Phlebotomus tobbi and Phlebotomus perniciosus, whereas Phlebotomus ariasi is evolutionarily more distinct species. We demonstrated that there is no significantdifference between the transcriptomes, proteomes or enzymatic properties of the salivary components of Addis Zemen and Melka Werer. Thus, the different epidemiology of VL in these Ethiopian foci cannot be attributed to the salivary gland

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    GJ - Diseases and animal vermin, veterinary medicine

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA13-05292S" target="_blank" >GA13-05292S: Phlebotomus orientalis: salivary proteins and the host antibody response</a><br>

  • Continuities

    S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    PLoS Neglected Tropical Diseases [online]

  • ISSN

    1935-2735

  • e-ISSN

  • Volume of the periodical

    8

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    19

  • Pages from-to

  • UT code for WoS article

    000332017500009

  • EID of the result in the Scopus database