Polymerase Synthesis of Photocaged DNA Resistant against Cleavage by Restriction Endonucleases

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10271756" target="_blank" >RIV/00216208:11310/14:10271756 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/14:00429964
Result on the web
<a href="http://dx.doi.org/10.1002/anie.201402370" target="_blank" >http://dx.doi.org/10.1002/anie.201402370</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/anie.201402370" target="_blank" >10.1002/anie.201402370</a>

Result language
angličtina
Original language name
Polymerase Synthesis of Photocaged DNA Resistant against Cleavage by Restriction Endonucleases
Original language description
5-[(2-Nitrobenzyl) oxymethyl]-2'-deoxyuridine 5'-O-triphosphate was used for polymerase (primer extension or PCR) synthesis of photocaged DNA that is resistant to the cleavage by restriction endonucleases. Photodeprotection of the caged DNA released 5-hydroxymethyluracil-modified nucleic acids, which were fully recognized and cleaved by restriction enzymes.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
—

Project
<a href="/en/project/GA14-04289S" target="_blank" >GA14-04289S: Modifications and bioorthogonal reactions in the major groove of DNA for regulation of protein binding and gene expression</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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