Polymerase Synthesis of DNAs Bearing Vinyl Groups in the Major Groove and their Cleavage by Restriction Endonucleases
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10286199" target="_blank" >RIV/00216208:11310/14:10286199 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/14:00435030
Result on the web
<a href="http://dx.doi.org/10.1002/cbic.201402319" target="_blank" >http://dx.doi.org/10.1002/cbic.201402319</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cbic.201402319" target="_blank" >10.1002/cbic.201402319</a>
Alternative languages
Result language
angličtina
Original language name
Polymerase Synthesis of DNAs Bearing Vinyl Groups in the Major Groove and their Cleavage by Restriction Endonucleases
Original language description
DNA molecules containing 5-vinyluracil, 5-vinylcytosine, or 7-deaza-7-vinyladenine were prepared by polymerase incorporation of the corresponding vinyl-modified 2-deoxyribonucleoside triphosphates, and the influence of the vinyl group in the major grooveof DNA on the cleavage by diverse type II restriction endonucleases (REs) was studied. The presence of 5-vinyluracil was tolerated by most of the REs, whereas only some REs were able to cleave sequences containing 7-deaza-7-vinyladenine. The enzyme ScaIwas found to cleave DNA containing 5-vinylcytosine efficiently but not DNA containing the related 5-ethynylcytosine. All other REs failed to cleave sequences containing any cytosine modifications.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA14-04289S" target="_blank" >GA14-04289S: Modifications and bioorthogonal reactions in the major groove of DNA for regulation of protein binding and gene expression</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chembiochem : a European journal of chemical biology
ISSN
1439-4227
e-ISSN
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Volume of the periodical
15
Issue of the periodical within the volume
15
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
2306-2312
UT code for WoS article
000342901800020
EID of the result in the Scopus database
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