Cleavage of DNA containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10315681" target="_blank" >RIV/00216208:11310/15:10315681 - isvavai.cz</a>

Alternative codes found

RIV/61388963:_____/15:00453449

Result on the web

<a href="http://dx.doi.org/10.1016/j.bmc.2015.09.051" target="_blank" >http://dx.doi.org/10.1016/j.bmc.2015.09.051</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bmc.2015.09.051" target="_blank" >10.1016/j.bmc.2015.09.051</a>

Result language

angličtina

Original language name

Cleavage of DNA containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases

Original language description

A systematic study of the cleavage of DNA sequences containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases (REs) was performed and the results compared with the same sequences containing natural pyrimidine bases, uracil or 5-methylcytosine. The results show that some REs recognize fluorine as a hydrogen on cytosine and cleave the corresponding sequences where the presence of m5dC leads to blocking of the cleavage. However, on uracil, the same REs recognize the F as a methyl surrogate and cleave the sequences which are not cleaved if uracil is incorporated instead of thymine. These results are interesting for understanding the recognition of DNA sequences by REs and for manipulation of the specific DNA cutting.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CC - Organic chemistry

OECD FORD branch

—

Project

<a href="/en/project/GA14-04289S" target="_blank" >GA14-04289S: Modifications and bioorthogonal reactions in the major groove of DNA for regulation of protein binding and gene expression</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bioorganic and Medicinal Chemistry

ISSN

0968-0896

e-ISSN

—

Volume of the periodical

23

Issue of the periodical within the volume

21

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

6885-6890

UT code for WoS article

000364437400011

EID of the result in the Scopus database

2-s2.0-84946141941