Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F11%3A00360993" target="_blank" >RIV/61388963:_____/11:00360993 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/cbic.201000644" target="_blank" >http://dx.doi.org/10.1002/cbic.201000644</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cbic.201000644" target="_blank" >10.1002/cbic.201000644</a>
Alternative languages
Result language
angličtina
Original language name
Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases
Original language description
A series of six pyrimidine modified dNTPs: 5-ethynyl- 5-phenyl- and 5-(3-nitrophenyl)-deoxycitidine and -deoxyuridine triphosphates were prepared and incorporated by primer extension using Vent (exo-) polymerase to specific DNA sequences within or next to the recognition sequences of selected restriction endonucleases. Then the cleavage of these pyrimidine-modified DNA sequences by 13 restriction enzymes was studied. While the presence of any modified C within the target sequence completely prevented any restriction cleavage, most enzymes tolerated the presence of 5-ethynylU and two of them even the presence of 5-phenyl- and 5-(3-nitrophenyl)U. Modifications outside the recognition sequence were tolerated apart from phenyl derivatives with PvuII enzyme. 5-EthynylC was used for protection of the recognition sequence from cleavage in presence of the second unmodified copy of the same sequence that was cleaved.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chembiochem
ISSN
1439-4227
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
3
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
431-438
UT code for WoS article
000287718700016
EID of the result in the Scopus database
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