Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F09%3A00334373" target="_blank" >RIV/61388963:_____/09:00334373 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases

Original language description

A set of base-modified 2'-deoxyadenosine derivatives was incorporated to diverse DNA sequences by primer extension and the influence of the modification on cleavage by diverse restriction endonucleases was studied. While 8-substituted adenine derivativeswere well tolerated by the enzymes, the presence of 7-substituted 7-deazaadenine in the recognition sequence resulted in blocking of cleavage by some enzymes depending on the nature and size of the 7-substituent. The results are useful both for protection of some sequences from cleavage and for manipulation of functionalized DNA by restriction cleavage.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CC - Organic chemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

37

Issue of the periodical within the volume

22

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

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UT code for WoS article

000272935000032

EID of the result in the Scopus database

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