Polymerase Synthesis and Restriction Enzyme Cleavage of DNA Containing 7-Substituted 7-Deazaguanine Nucleobases
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10315455" target="_blank" >RIV/00216208:11310/15:10315455 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/15:00451292
Result on the web
<a href="http://dx.doi.org/10.1002/cbic.201500315" target="_blank" >http://dx.doi.org/10.1002/cbic.201500315</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cbic.201500315" target="_blank" >10.1002/cbic.201500315</a>
Alternative languages
Result language
angličtina
Original language name
Polymerase Synthesis and Restriction Enzyme Cleavage of DNA Containing 7-Substituted 7-Deazaguanine Nucleobases
Original language description
Previous studies of polymerase synthesis of base-modified DNAs and their cleavage by restriction enzymes have mostly related only to 5-substituted pyrimidine and 7-substituted 7-deazaadenine nucleotides. Here we report the synthesis of a series of 7-substituted 7-deazaguanine 2'-deoxyribonucleoside 5'-O-triphosphates (dGRTPs), their use as substrates for polymerase synthesis of modified DNA and the influence of the modification on their cleavage by type II restriction endonucleases (REs). The dGRTPs were generally good substrates for polymerases but the PCR products could not be visualised on agarose gels by intercalator staining, due to fluorescence quenching. The presence of 7-substituted 7-deazaguanine residues in recognition sequences of REs in most cases completely blocked the cleavage.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GA14-04289S" target="_blank" >GA14-04289S: Modifications and bioorthogonal reactions in the major groove of DNA for regulation of protein binding and gene expression</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chembiochem : a European journal of chemical biology
ISSN
1439-4227
e-ISSN
—
Volume of the periodical
16
Issue of the periodical within the volume
15
Country of publishing house
DE - GERMANY
Number of pages
12
Pages from-to
2225-2236
UT code for WoS article
000363422000016
EID of the result in the Scopus database
2-s2.0-84943352901