Cadmium-induced cell death in BY-2 cell culture starts with vacuolization of cytoplasm and terminates with necrosis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10271981" target="_blank" >RIV/00216208:11310/14:10271981 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/ppl.12124" target="_blank" >http://dx.doi.org/10.1111/ppl.12124</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/ppl.12124" target="_blank" >10.1111/ppl.12124</a>

Result language

angličtina

Original language name

Cadmium-induced cell death in BY-2 cell culture starts with vacuolization of cytoplasm and terminates with necrosis

Original language description

Cadmium is a potent inducer of programmed cell death (PCD) in plants but the morphological changesin cells exposed to cadmium are poorly characterized. Using light and transmission electron microscopy (TEM) we have investigated the changes in ultrastructure of tobacco BY-2 cells treated with 50 ?M CdSO4. The cadmium-induced alterations in cell morphology occurred gradually over a period of 3-4 days and the first stages of the response resembled vacuolar type of cell death. The initial formation of numerous small cytoplasmic vacuoles and dilation of endoplasmic reticulum was followed first by fusion of smaller vacuoles with each other and with big vacuoles, and then by the appearance of autophagic vacuoles containing autophagic bodies. The final stagesof cell death were accompanied by necrotic features including loss of plasmalemma integrity, shrinkage of the protoplast and unprocessed cellular components. In addition, we observed a gradual degradation of nuclear material. Our results

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

<a href="/en/project/LC06034" target="_blank" >LC06034: Regulation of morphogenesis of plant cells and organs</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiologia Plantarum

ISSN

0031-9317

e-ISSN

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Volume of the periodical

151

Issue of the periodical within the volume

4

Country of publishing house

DK - DENMARK

Number of pages

11

Pages from-to

423-433

UT code for WoS article

000340253700005

EID of the result in the Scopus database

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