The effect of aristolochic acid I on expression of NAD(P)H:quinone oxidoreductase in mice and rats-A comparative study

Result code in IS VaVaI

RIV/00216208:11310/14:10282870 - isvavai.cz

Result on the web

http://dx.doi.org/10.1016/j.mrgentox.2014.01.012

DOI - Digital Object Identifier

10.1016/j.mrgentox.2014.01.012

Result language

angličtina

Original language name

The effect of aristolochic acid I on expression of NAD(P)H:quinone oxidoreductase in mice and rats-A comparative study

Original language description

Aristolochic acid is the cause of aristolochic acid nephropathy (AAN) and Balkan endemic nephropathy (BEN) and their associated urothelial malignancies. Using Western blotting, we investigated the expression of NAD(P)H:quinone oxidoreductase (NQO1), themost efficient cytosolic enzyme that reductively activates aristolochic acid I (AAI) in mice and rats. In addition, the effect of AAI on the expression of the NQO1 protein and its enzymatic activity in these experimental animal models was examined. We found that NQO1 protein levels in cytosolic fractions isolated from liver, kidney and lung of mice differed from those expressed in these organs of rats. In mice, the highest levels of NQO1 protein and NQO1 activity were found in the kidney, followed by lung and liver. In contrast, the NQO1 protein levels and enzyme activity were lowest in rat-kidney cytosol, whereas the highest amounts of NQO1 protein and activity were found in lung cytosols, followed by those of liver. NQO1 protein and e

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

GA303/09/0472: Study on participation of biotransformation enzymes in development of renal injury and urothelial cancer mediated by aristolochic acid

Continuities

S - Specificky vyzkum na vysokych skolach
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

ISSN

1383-5718

e-ISSN

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Volume of the periodical

768

Issue of the periodical within the volume

JUL 1 2014

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

1-7

UT code for WoS article

000337883400001

EID of the result in the Scopus database

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