Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser-Induced Fluorescence Detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10283299" target="_blank" >RIV/00216208:11310/14:10283299 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/14:00230438 RIV/00216208:11130/14:10283299 RIV/00216305:26620/14:PU112643 RIV/00064203:_____/14:10283299

Result on the web

<a href="http://dx.doi.org/10.1007/s10337-014-2733-6" target="_blank" >http://dx.doi.org/10.1007/s10337-014-2733-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10337-014-2733-6" target="_blank" >10.1007/s10337-014-2733-6</a>

Result language

angličtina

Original language name

Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser-Induced Fluorescence Detection

Original language description

Doxorubicin (DOX) belongs to the group of anthracycline antibiotics with very effective anticancer properties. On the other hand, the cardiotoxic effects limit its application over the maximum cumulative dose. To overcome this obstacle, encapsulation ofthis drug into the protective nanotransporter such as apoferritin is beneficial. In this study, fluorescent behavior of DOX in various solvents was determined by fluorescence spectrometry, demonstrating the fluorescence quenching effect of water, which is often used as a solvent. It was found that by increasing the amount of the organic phase in the DOX solvent the dynamic quenching is significantly suppressed. Ethanol, acetonitrile and dimethyl sulfoxide were tested and the best linearity of the calibration curve was obtained when above 50 % of the solvent was present in the binary mixture with water. Moreover, pH influence on the DOX fluorescence was also observed within the range of 4-10. Two times higher fluorescence intensity was o

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GAP301%2F10%2F0356" target="_blank" >GAP301/10/0356: Study of contribution of different DNA-damaging mechanisms to toxicity of cytostatics to human chemosensitive and chemoresistant neuroblastomas</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chromatographia

ISSN

0009-5893

e-ISSN

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Volume of the periodical

77

Issue of the periodical within the volume

21-22

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

1469-1476

UT code for WoS article

000345004800008

EID of the result in the Scopus database

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