Enhancement of sensitivity in separation techniques with fluorescence detection using deuterated water
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00103114" target="_blank" >RIV/00216224:14310/18:00103114 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Enhancement of sensitivity in separation techniques with fluorescence detection using deuterated water
Original language description
Fluorescent molecules with emission maxima above 500 nm are often dynamically quenched by OH oscillators present in water molecules. We have changed H2O for D2O to obtain higher fluorescence intensity signal. Our experiments carried out on spectrofluorometer have proved that anthracyclines can achieve about 300 % higher response in D2O as solvent. The affect can be employed in various analytical methods including HPLC and capillary electrophoresis coupled to fluorescence detection.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů