Enhancement of luminescence signal by deuterated water - Practical implications
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F22%3A00119391" target="_blank" >RIV/00216224:14310/22:00119391 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/22:00551533
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0925400521015975?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0925400521015975?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.snb.2021.131029" target="_blank" >10.1016/j.snb.2021.131029</a>
Alternative languages
Result language
angličtina
Original language name
Enhancement of luminescence signal by deuterated water - Practical implications
Original language description
The fluorescence signal increase in deuterated water - D2O is relevant to many methods used in chemical and biological research. We performed fluorescence spectroscopy measurements combined with evaluations of fluorescence-based methods to describe and quantify the practical impacts of D2O on chemical and biological samples. Several novel features are observed in fluorescence spectra and life-time decay curves of molecules including fluorescence probes and labels that are used frequently in biomolecular labelling and bioimaging. The observed characteristics suggest that D2O enhances the fluorescence signal due to the exchange of labile hydrogen on a fluorophore for heavier deuteron and the reduction of energy transfer from excited fluorophores to H2O molecules. Furthermore, performed quantifications approve that D2O increases significantly the detection signal of all examined fluorescence-based approaches where the brightness of fluorophores and sample viability are crucial.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Sensors and Actuators B: Chemical
ISSN
0925-4005
e-ISSN
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Volume of the periodical
352
Issue of the periodical within the volume
1
Country of publishing house
CH - SWITZERLAND
Number of pages
8
Pages from-to
1-8
UT code for WoS article
000721027600003
EID of the result in the Scopus database
2-s2.0-85118845933