Fluorophore Multimerization as an Efficient Approach towards Bright Protein Labels
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F21%3A00542326" target="_blank" >RIV/61388963:_____/21:00542326 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1002/ejoc.202100117" target="_blank" >https://doi.org/10.1002/ejoc.202100117</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/ejoc.202100117" target="_blank" >10.1002/ejoc.202100117</a>
Alternative languages
Result language
angličtina
Original language name
Fluorophore Multimerization as an Efficient Approach towards Bright Protein Labels
Original language description
Cell analysis techniques like flow cytometry and fluorescence microscopy are widely used to explore cells in real‐time and provide important insights into a variety of cellular processes. These techniques require bright and specific staining reagents to generate strong fluorescence signal and enable reliable read‐out, making the choice of fluorescent labels a key aspect of experimental design. Much research has been done in the field of fluorophore development to generate bright small‐molecule dyes, fluorescent proteins, and emitting nanoparticles. However, it remains challenging to modify biomolecules with multiple emitters and avoid self‐quenching of such fluorophores at the same time. Herein, we present advances in multimerization‐based methods for the generation of fluorescent labels with high brightness and discuss their advantages and limitations in the context of flow cytometry and fluorescence microscopy applications.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10401 - Organic chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal of Organic Chemistry
ISSN
1434-193X
e-ISSN
1099-0690
Volume of the periodical
2021
Issue of the periodical within the volume
20
Country of publishing house
DE - GERMANY
Number of pages
14
Pages from-to
2817-2830
UT code for WoS article
000644543900001
EID of the result in the Scopus database
2-s2.0-85104988223