The Application of an Emerging Technique for Protein-Protein Interaction Interface Mapping: The Combination of Photo-Initiated Cross-Linking Protein Nanoprobes with Mass Spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10283840" target="_blank" >RIV/00216208:11310/14:10283840 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/14:00440657
Result on the web
<a href="http://dx.doi.org/10.3390/ijms15069224" target="_blank" >http://dx.doi.org/10.3390/ijms15069224</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms15069224" target="_blank" >10.3390/ijms15069224</a>

Result language
angličtina
Original language name
The Application of an Emerging Technique for Protein-Protein Interaction Interface Mapping: The Combination of Photo-Initiated Cross-Linking Protein Nanoprobes with Mass Spectrometry
Original language description
Protein-protein interaction was investigated using a protein nanoprobe capable of photo-initiated cross-linking in combination with high-resolution and tandem mass spectrometry. This emerging experimental approach introduces photo-analogs of amino acidswithin a protein sequence during its recombinant expression, preserves native protein structure and is suitable for mapping the contact between two proteins. The contact surface regions involved in the well-characterized interaction between two moleculesof human 14-3-3 zeta regulatory protein were used as a model. The employed photo-initiated cross-linking techniques extend the number of residues shown to be within interaction distance in the contact surface of the 14-3-3 lambda dimer (Gln8-Met78). Theresults of this study are in agreement with our previously published data from molecular dynamic calculations based on high-resolution chemical cross-linking data and Hydrogen/Deuterium exchange mass spectrometry. The observed contact is
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
<a href="/en/project/GAP207%2F12%2F0627" target="_blank" >GAP207/12/0627: MAMMALIAN MICROSOMAL CYTOCHROME P450 INTERACTION WITH REDOX PARTNERS - TOPOLOGY AND STRUCTURE-FUNCTION RELATIONSHIPS</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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