Mapping protein structural changes by quantitative cross-linking
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10294866" target="_blank" >RIV/00216208:11310/15:10294866 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/15:00455585
Result on the web
<a href="http://dx.doi.org/10.1016/j.ymeth.2015.05.027" target="_blank" >http://dx.doi.org/10.1016/j.ymeth.2015.05.027</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ymeth.2015.05.027" target="_blank" >10.1016/j.ymeth.2015.05.027</a>
Alternative languages
Result language
angličtina
Original language name
Mapping protein structural changes by quantitative cross-linking
Original language description
Chemical cross-linking is a promising technology for protein tertiary structure determination. Though the data has low spatial resolution, it is possible to obtain it at physiological conditions on proteins that are not amenable to standard high resolution techniques such as X-ray, NMR analysis and cryo-EM. Here we demonstrate the utilization of isotopically labeled chemical cross-linking to visualize protein conformation rearrangements. Since calmodulin exists in two distinct conformations (calcium-free and calcium-containing forms), we selected this protein for testing the potential and the limits of a new technique. After cross-linking of both calmodulin forms, the calcium-free and calcium-containing forms were mixed together and digested under different conditions and the products of proteolysis were monitored using high resolution mass spectrometry. Finally, the ratios of heavy/light cross-links were calculated by mMass open source platform.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Methods
ISSN
1046-2023
e-ISSN
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Volume of the periodical
89
Issue of the periodical within the volume
1 November 2015
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
112-120
UT code for WoS article
000365062800014
EID of the result in the Scopus database
2-s2.0-84946405388