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Novel Functions of an Iron-Sulfur Flavoprotein from Trichomonas vaginalis Hydrogenosomes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10285600" target="_blank" >RIV/00216208:11310/14:10285600 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388963:_____/14:00430460

  • Result on the web

    <a href="http://dx.doi.org/10.1128/AAC.02320-13" target="_blank" >http://dx.doi.org/10.1128/AAC.02320-13</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1128/AAC.02320-13" target="_blank" >10.1128/AAC.02320-13</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Novel Functions of an Iron-Sulfur Flavoprotein from Trichomonas vaginalis Hydrogenosomes

  • Original language description

    Iron-sulfur flavoproteins (Isf) are flavin mononucleotide (FMN)- and FeS cluster-containing proteins commonly encountered in anaerobic prokaryotes. However, with the exception of Isf from Methanosarcina thermophila, which participates in oxidative stressmanagement by removing oxygen and hydrogen peroxide, none of these proteins has been characterized in terms of function. Trichomonas vaginalis, a sexually transmitted eukaryotic parasite of humans, was found to express several iron-sulfur flavoprotein (TvIsf) homologs in its hydrogenosomes. We show here that in addition to having oxygen-reducing activity, the recombinant TvIsf also functions as a detoxifying reductase of metronidazole and chloramphenicol, both of which are antibiotics effective againsta variety of anaerobic microbes. TvIsf can utilize both NADH and reduced ferredoxin as electron donors. Given the prevalence of Isf in anaerobic prokaryotes, we propose that these proteins are central to a novel defense mechanism against

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GC13-09208J" target="_blank" >GC13-09208J: Molecular mechanisms of Trichomonas vaginalis infection: role of energy metabolism</a><br>

  • Continuities

    S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Antimicrobial Agents and Chemotherapy

  • ISSN

    0066-4804

  • e-ISSN

  • Volume of the periodical

    58

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    9

  • Pages from-to

    3224-3232

  • UT code for WoS article

    000338776900030

  • EID of the result in the Scopus database