Purification and enzymatic characterization of tobacco leaf ?-N-acetylhexosaminidase
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10286865" target="_blank" >RIV/00216208:11310/14:10286865 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.biochi.2014.09.006" target="_blank" >http://dx.doi.org/10.1016/j.biochi.2014.09.006</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.biochi.2014.09.006" target="_blank" >10.1016/j.biochi.2014.09.006</a>
Alternative languages
Result language
angličtina
Original language name
Purification and enzymatic characterization of tobacco leaf ?-N-acetylhexosaminidase
Original language description
The kinetic properties of ?-N-acetylhexosaminidase purified from tobacco (Nicotiana tabacum L.) leaves have been investigated. In addition to chromogenic pNP derivates, N,N'-diacetylchitobiose and N,N',N"-triacetylchitotriose were also used as substratesof ?-N-acetylhexosaminidase. The highest reaction rate and the affinity for the substrate were observed for pNP-GlcNAc; however, an excess of this substrate inhibits the reaction. The reaction rate with pNP-GalNAc as the substrate was found to be about85% of that obtained with pNP-GlcNAc. The hydrolysis of acetylated chitooligomers by ?-N-acetylhexosaminidase followed by separation and quantification using capillary electrophoresis was slower compared to pNP-GlcNAc. The pH optimum of ?-N-acetylhexosaminidase for individual substrates was found at 4.3-5.0 and the temperature optimum was 50-55 oC. Gel permeation chromatography and red native electrophoresis determined the relative molecular weight as 280 000 and the isoelectric point as
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/1M0505" target="_blank" >1M0505: Center of targeted therapeutic drugs</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biochimie
ISSN
0300-9084
e-ISSN
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Volume of the periodical
107 Part B
Issue of the periodical within the volume
December 2014
Country of publishing house
FR - FRANCE
Number of pages
7
Pages from-to
263-269
UT code for WoS article
000347742400011
EID of the result in the Scopus database
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