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Applications of Capillary Electrophoresis in Enzyme Assays

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10172702" target="_blank" >RIV/00216208:11310/13:10172702 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.bestservis.eu/upload/file/Sbornik_2013.pdf" target="_blank" >http://www.bestservis.eu/upload/file/Sbornik_2013.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Applications of Capillary Electrophoresis in Enzyme Assays

  • Original language description

    In this work, we present several applications that manifest the potential of capillary electrophoresis in enzyme assays. Capillary electrophoresis was employed to perform offline and online assays of beta-N-acetylhexosaminidase for three different substrates. Offline assays were successfully utilized to monitor the enzyme reaction and to determine parameters such as pH and temperature optimum. The offline assay was also used to compare activities of the enzyme towards the natural and chromogenic substrates. Finally, an online enzyme assay was developed and proved to be highly beneficial for inhibitor screening.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Sborník přednášek mezinárodní odborné konference XXXIII. Moderní Elektrochemické Metody Jetřichovice 20. - 24. května 2013

  • ISBN

    978-80-905221-1-4

  • ISSN

  • e-ISSN

  • Number of pages

    5

  • Pages from-to

    96-100

  • Publisher name

    Srsenová Lenka - BEST servis Ústí nad Labem

  • Place of publication

    Ústí nad Labem

  • Event location

    Jetřichovice, Czech Republic

  • Event date

    May 20, 2013

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article